Death toll from U.S.-led strike rises to 52 civilians on the northern Syrian province of Aleppo including seven children, activists on the ground monitoring the conflict said on Saturday.

Activists said the death toll from Friday’s strike was the highest civilian loss in a single attack by U.S. and Arab forces since they started air raids against hardline Islamist militant groups in Syria such as Islamic State.

U.S.-led forces are also targeting the group in Iraq.

The activists on the ground in Syria said the raid had mistakenly struck civilians in a village on the eastern banks of the Euphrates River in Aleppo province, killing members of at least six families.

U.S.-led strikes had killed at least 66 civilians in Syria from the start of the raids on Sept. 23 until Friday’s strike, which brought the total to at least 118. The campaign has also killed nearly 2,000 Islamic State fighters.

The activists said at least 13 people were still missing from Friday’s raid.

The U.S. military did not confirm the civilian deaths but said it takes all such reports seriously and would look into the matter further.

“We currently have no information to corroborate allegations that coalition air strikes resulted in civilian casualties,” Major Curt Kellogg, spokesman for U.S. Central Command, said in an email.

The U.S.-led air strikes have had little impact on the hardline Islamic State group, slowing its advances but failing to weaken it in areas it controls. The group has built its own government in Syria’s city of Raqqa, where it is most powerful.

Washington and its allies say their aim is to support what they call moderate rebels fighting against both Syrian President Bashar al-Assad and Islamic State.

But four years into Syria’s civil war, no side is close to victory. A third of the population has been made homeless and more than 220,000 people have been killed.

Government forces have seen a series of setbacks on the battlefield recently and Islamist fighters have edged closer to Assad’s stronghold in the coastal areas.

Fighting continued on Saturday between government forces and Islamist fighters in government-held Latakia, heartland of Assad’s minority Alawite community.

The violence follows advances in neighboring Idlib province by the hardline Ahrar al-Sham group and Syria’s al Qaeda wing Nusra Front, as well as other allied fighters.

Syria’s state news agency said the army carried out overnight strikes on Nusra positions in Idlib.

Reuters contributed to this article.

Death toll from U.S.-led strike rises to 52 civilians in Syria

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